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|Year : 2013
: 15 | Issue : 64 | Page
|Effect of administration of vitamins C and E on fertilization capacity of rats exposed to noise stress
Ghasem Saki1, Majid Jasemi2, Ali Reza Sarkaki1, Ali Fathollahi2
1 Physiology Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2 Department of Urology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
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|Date of Web Publication||21-May-2013|
The aims of this study were to evaluate the effects of administration of Vitamins C and E on fertilization capacity in rats exposed to noise stress. 40 adult male rats were randomly divided into 5 equal groups. Group 1 as controls who were not exposed to noise and groups 2-5 exposed to noise with 90-120 dB intensity and 300-350 Hz frequency from 7 pm to 7 am everyday for 50 days. Group 2 exposed to noise and did not receive Vitamins. Group 3 received vitamin C, Group 4 received Vitamin E. Group 5 received Vitamins C and E concomitantly. After 50 days, serum Follicle-stimulating hormone (FSH), Luteinizing hormone (LH) and testosterone were calculated. Then each rat was left with three female rats for mating. Pregnant females were sacrificed on the 19 th day of pregnancy and evaluated for the presence and number of viable, dead and absorbed fetuses. The level of FSH, LH and testosterone significantly decreased in rats exposed to noise (P < 0.05). By administration of Vitamins in groups 3-5 we observed that the level of hormones significantly increased in compared to group 2 (P < 0.05). The fertilization capacity of male rats in groups 3-5 significantly increased in compared to group 2 (P < 0.05). There was significant difference between groups 1 and 2 in case of fertilization capacity (P = 0.001). The data in this study strongly suggests a negative role for noise stress on level of FSH, LH and testosterone level and also fertilization capacity of male rats. To complement the information it is suggested that this research be done on human samples.
Keywords: Antioxidants, infertility, mortality, pregnancy
|How to cite this article:|
Saki G, Jasemi M, Sarkaki AR, Fathollahi A. Effect of administration of vitamins C and E on fertilization capacity of rats exposed to noise stress. Noise Health 2013;15:194-8
|How to cite this URL:|
Saki G, Jasemi M, Sarkaki AR, Fathollahi A. Effect of administration of vitamins C and E on fertilization capacity of rats exposed to noise stress. Noise Health [serial online] 2013 [cited 2020 Jan 19];15:194-8. Available from: http://www.noiseandhealth.org/text.asp?2013/15/64/194/112374
| Introduction|| |
Infertility cases are about 20% due entirely to male factors, with an additional 30-40% of cases related to both male and female factors. , One of the most important aims is the evaluation of the infertile man to recognize reversible conditions, which are responsible for infertility. Exposure to chemical toxicants, stress, heat and cigarette smoking may cause infertility. , On the other hand, regarding to various effects of noise on different systems, the exact effect of this stress on fertility is yet to be elucidated. Geber had mentioned that the terato-genic action of noise is primarily the result of decreased utero-placental blood flow resulting in fetal hypoxia and increased secretion of maternal catecholamine. , and he noticed that in pregnant rats exposed to noise stress the litter size and the number of resorption per litter significantly have been reduced.  Previous study had shown that the stress like noise and forced swimming stress caused decreased in sperm count and motility and increased pre-implantation mortality, decreased litter size, embryo size and weight among the exposed offspring. ,,,, However, the data are inconsistent among the various experimental conditions. ,,,, As we know the antioxidants are the main defense against oxidative stress induced by free radicals. There are prevention antioxidants such as metal chelators and metal-binding proteins block the formation of new free radicals and scavenger antioxidants, which remove the free radicals that have already formed.  Oxidative stress may be limited by using chain-breaking antioxidants such as vitamin E and vitamin C as drug supplements.  More specifically speaking, these vitamins have been shown to have protective effect on testis and fertility. , This study was design to evaluate the effect of administration of vitamin C and vitamin E on fertilization capacity of rats exposed to noise stress.
| Methods|| |
This experimental study was performed in the physiology research center from September 2010 to December 2010. Thirty-two male rats of the Wistar strain weighing 220 ± 20 g were used for the experiments. The animals were acclimated to 22 ± 1°C and maintained under conditions of 12-h periods of light and dark, with free access to tap water and commercial rat food. All procedures were approved by international guidelines and by the Institute Research Ethics and Animal Care and use committee of Ahvaz Jundishapur University of Medical Sciences. Every effort was made to minimize the number of animals used and their suffering.
Forty adult male rats were randomly divided into 5 equal groups. Group 1 as control were not exposed to noise and groups 2-5 exposed to noise with 90-120 dB intensity and 300-350 Hz frequency from 7 pm to 7 am every day for 50 days. Group 2 exposed to noise and did not receive Vitamins. Group 3 received vitamin C (125 mg/kg/day), group 4 received vitamin E (75 mg/kg/day). Group 5 received Vitamins C and E concomitantly.  For this to be done, these groups were transported to the room which had dimensions of 3 × 4 × 3 meter and was lagged by wood and acoustic segments (anti-loud voice). In this room, the set of WHITE NOISE which produce noise was prepared at 19o'clock in the case of the frequency of 300-350Hz and intensity of 90-120dB.  and the set timer was arranged in a way that after 1 h of operation and producing noise by the speaker, it would turn off for a few minutes (From 15 min to 60 min), before it operates again. It has to be mentioned that, this would prevent animal's compatibility. The noise producing device changed the intensity and frequency of noise produced automatically in the district of minimum and maximum every 2-3 min, and this also aided in non-compatibility.  For evaluating the amount and intensity of noise, we used noise level meter and thus rate and intensity was controlled in this way.  Group 2 received vitamin C. Group 3 received vitamin E (75 mg/kg/day) Group 4 received Vitamins C and E concomitantly. After 50 days, blood sample was drawn from each rat's tail and sent to laboratory for serum Follicle-stimulating hormone (FSH), Luteinizing hormone (LH) and testosterone measurement. These determinations were done with Elisa technique. After that time, each rat was left with three female rats for mating. Every morning, females with positive vaginal plaques were identified and separated. Pregnant females were killed on the 19 th day of pregnancy by chloroform inhalation. Their uteri were then evaluated with regard to the presence and number of viable, dead and absorbed fetuses. To weigh the live embryos, a certain amount of water was poured and weighted then a fetus was spilled into the water. Finally, the weight difference of the two numbers, birth weight was achieved.
Data are reported as mean ± SD and percentage. The statistical significance of difference between the control and other groups was determined by ANOVA test for hormonal studies. Comparison of pregnancy rate and the number of dead/absorbed fetuses were determined by Chi-square test. Differences between the means were considered to be significant when P < 0.05 was achieved.
| Results|| |
The concentrations of FSH, LH and testosterone in plasma of male rates exposed/non-exposed to noise stress showed in [Table 1]. We observed that the levels of FSH in non-exposed to noise rats is 21.56 ± 1.07 and in exposed to noise rats is 10.48 ± 1.14. Statistical analysis showed that the difference between two groups was significant (P < 0.05). The concentrations of FSH in groups 3-5 were 18.12 ± 1.23, 17.46 ± 1.47 and 19.66 ± 1.92 respectively. The differences between groups 3 and5 were not significant (P > 0.05) but in compared to group 2 the differences were significant (P < 0.05).Levels of LH were 24.37 ± 1.27, 12.16 ± 1.23, 20.61 ± 1.07, 19.19 ± 1.11 and 18.90 ± 1.07 in groups 1 to 5 respectively. The level of LH in group 2 were lowest in compared to control and groups 3 to 5 (P < 0.05). The differences between groups 3 and 5 were not significant (P < 0.05). As shown in [Table 1], the levels of testosterone in groups 3-5 significantly decreased in compared to control and groups 5 (P < 0.05). There was no statistical difference between the two groups (Control and group 5) in terms of testosterone level (P = 0.07).
|Table 1: Plasma level of Follicle‑stimulating hormone, Luteinizing hormone and testosterone hormones in different groups of study|
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As shown in [Table 2], the pregnancy rate in female mated with control and noise exposed male was 21:24 (87.5%) and 6:24 (25%) respectively. Statistical analysis showed the significant differences between two groups (P < 0.05).Fertilization capacity of rats receiving Vitamins significantly increased in compared to non-treated group (P < 0.05). Present data showed that in the group treated concomitance with Vitamins C and E significantly increased in case of fertilization capacity (P < 0.05).The number of corpora lutea and also live fetuses in uterus of female mated with noise exposed males significantly decreased (P < 0.05).The body weight of fetus and number of dead fetus significantly decreased and resorption sites significantly increased in group 2 (P > 0.05).
|Table 2: Fertilization capacity and subsequent off spring quality in different groups of study|
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| Discussion|| |
Noise decreased serum testosterone level in our study, but supplementing rats with either vitamin C or E or the combination of both resulted in testosterone turning back to the normal range. This result is similar to a study by previous studies in which male albino rats were exposed to 100 dB of noise for 1 h and 3 h in acute group and daily 1 h exposure for 60 days, and 90 days in the chronic group. In that study, they could also show significant reduction in serum testosterone. , However, interestingly, acute noise stress of 80 dB increased the testosterone level. There is a decrease in testosterone secretion in male rats exposed to immobilization-induced stress, forced swimming stress, heat exposure-induces stress. ,,, In this study, noise increased serum FSH levels. Adding vitamin E or Vitamins C and E to their regimen, noise exposed rats were protected from noise's effect on FSH level. On the other hand, vitamin C alone could not compensate for negative effect of noise stress.
Serum LH level was also affected negatively by noise to an extent that vitamin C or E could not reverse the effect. However, the combination of Vitamins C and E had a protective effect against noise stress. In another study by Kimmel et al., pregnant mice were exposed to 100 dB of noise on days 3-6, 7-10, or 11-14 of gestation.  Significantly increased resorption rates and decreased number of live fetuses per litter were observed in each of the treated groups of animals. We also reached the same results as the number of pregnant rats containing any number of dead and/or absorbed fetuses was higher in those which had mated with noise exposed male rats. In accordance with this finding Saki et al. (2010) previously reported that the forced swimming stress had a harmful effect on fertilization capacity of rat and subsequent offspring quality.  Moreover, in females mating with noise expose males who had received Vitamins either alone or in combination, this untoward occurrence was corrected. Nawrot and colleague  exposed mated female mice to either semi-continuous 126 dB low-frequency noise, intermittent 110 dB mid-frequency noise, or semi-continuous, very high frequency (18-20 kHz) 113 dB noise on days 1-6 or 6-15 of gestation. Significantly increased embryo and fetal mortality, decreased fetal weight, and decreased pregnancy rate were reported among the exposed animals. Noise's negative effect was also reported by cosa and co-worker.  They showed abnormalities in reproduction and a significant decrease in pregnancy rate and lethal effect in mice embryos exposed to high frequencies of noise Once again in our study and on assessing pregnancy rate, overall, there was not any significant difference; but when only rats in control group were compared with noise exposed rats the difference became meaningful. This is in line with previous results.
In a follow-up study, Nawrot et al.  exposed mated female mice to high-frequency 110 dB noise on days 6-15 of gestation. Decreased pregnancy rate and mean fetal weight and increased fetal mortality were observed among the exposed animals. In contrast, we did find noise stress to be predictable of increased occurrence of abnormal pregnancy outcome defined as fetal resorption and/or death. On the other hand, vitamin supplementation could potentially prevent them. One might translate this difference as more teratogenic or detrimental effect of noise stress on male fertility rather than on female reproductively.
Vitamin C neutralizes hydroxyl, superoxide, and hydrogen peroxide radicals and prevents sperm agglutination.  In addition, it also helps recycle vitamin E.  Lewis et al.  found vitamin C in reduced quantityin the seminal plasma of infertile men .In a study by Dawson et al.  vitamin C increased sperm counts in vivo in infertile malepatients. Akmal et al.  showed that vitamin C supplementation in infertile men might improve sperm count, sperm motility, and sperm morphology. Moving along with previous studies, we demonstrated that vitamin C had a protective effect on fertility rate and fetal abortion and death; however, and of interest was that its effect on FSH and LH was not significant.
In a randomized cross-over study, vitamin E improved sperm function as assessed by the zona binding test.  In our study, vitamin E could avert the negative effect of noise regarding pregnancy rate and the rate of fetal death and abortion. Like vitamin C, it had no protective effect on the level of LH, but FSH level was improved. Likewise, combination of vitamin C and E made pregnancy rate and the rate of fetal abortion and death normal. Of importance was that the combination rendered FSH and LH levels normal. However, according to a literature review by Agarwal et al.  many studies have failed to examine the effect of antioxidants on a specific group of infertile patients with high oxidative stress. Lastly, we did not perform a histological evaluation, but taking into account the huge changes in hormonal milieu it may be conferred that noise would cause structural changes leading to abnormalities in FSH, LH and testosterone levels. Our data is in contrast with a study by Gunther  who examined the effect of noise on the fertility of 21 male Guinea pigs. Sounding of 110 dB lasted 3-5 h daily over a period of 22-31 days. Histological examination of the testicular tissue did not show any disorder of spermatogenesis. Nevertheless, he believed that the negative result was attributed to failings in the test arrangement. Sounding only lasted for a maximum of 31 days. However, the duration of spermatogenesis in Guinea pigs is about 40 days. Moreover, the sounding maximum was only 5 h per day, yet the time of recovery took 4-5 times longer every day. Consequently, the daily short-term depression of the neuroendrocrinium affected the gonadotropin secretion in similarity to the daily biorhythmic variation. Therefore the expected inhibition of fertility by the neuroendrocrinic way could not be demonstrated. 
| Conclusion|| |
The data in this study strongly suggests a negative role for noise stress on fertility of the male rats. These results, however, should be further assessed on humans (e.g., factory workers, those living near airports, etc.). It is also recommended that the effect of noise on testicular histology be evaluated in other studies.
| Acknowledgments|| |
This paper is issued from thesis of Ali Fathollahi and the financial support was provided by Ahvaz Jundishapur University of Medical Sciences.
| References|| |
|1.||Mosher WD, Pratt WF. Fecundity and infertility in the United States: Incidence and trends. FertilSteril 1991;56:192-3. |
|2.||Thonneau P, Marchand S, Tallec A, Ferial ML, Ducot B, Lansac J, et al. Incidence and main causes of infertility in a resident population (1,850,000) of three French regions (1988-1989). Hum Reprod 1991;6:811-6. |
|3.||Ahmadnia H, Ghanbari M, Moradi MR, Khaje-Dalouee M. Effect of cigarette smoke on spermatogenesis in rats. Urol J 2007;4:159-63. |
|4.||Erat M, Ciftci M, Gumustekin K, Gul M. Effects of nicotine and vitamin E on glutathione reductase activity in some rat tissues in vivo and in vitro. Eur J Pharmacol 2007;554:92-7. |
|5.||Geber WF. Developmental effects of chronic maternal audiovisual stress on the rat fetus. J Embryol Exp Morphol 1966;16:1-16. |
|6.||Geber W. Cardiovascular and teratogenic effects of chronic intermittent noise stress. In: Welch BL, Welch AS, editors. Physiological Effects of Noise. New York: Plenum Press; 1995. p. 88-90. |
|7.||Zakem HB, Alliston CW. The effects of noise level and elevated ambient temperatures upon selected reproductive traits in female Swiss-Webster mice. Lab Anim Sci 1974;24:469-75. |
|8.||Saki G, Rahim F, Alizadeh K. Effect of forced swimming stress on count, motility and fertilization capacity of the sperm in adult rats. J Hum Reprod Sci 2009;2:72-5. |
|9.||Saki G, Razie S, Amirpoor S. Pregnancy rate in female mice exposed to forced swimming stress. Asian J Biol Sci 2011;4:266-71. |
|10.||Saki G, Rahim F, Vaysi OA. Effect of forced swimming stress on in-vivo fertilization capacity of rat and subsequent offspring quality. J Hum Reprod Sci 2010;3:32-4. |
|11.||Jalali M, Saki G, Sarkaki AR, Karami K, Nasri S. Effect of noise stress on count, progressive and non-progressive sperm motility, body and genital organ weights of adult male rats. J Hum Reprod Sci 2012;5:48-51. |
|12.||Kimmel CA, Cook RO, Staples RE. Teratogenic potential of noise in mice and rats. Toxicol Appl Pharmacol 1976;36:239-45. |
|13.||Nawrot PS, Cook RO, Staples RE. Embryotoxicity of various noise stimuli in the mouse. Teratology 1980;22:279-89. |
|14.||Cosa M, Cosa G. Annoyance, disturbance and damage caused by noise and vibrations. Ann Ig 1989;1:133-56. |
|15.||Cook RO, Nawrot PS, Hamm CW. Effects of high-frequency noise on prenatal development and maternal plasma and uterine catecholamine concentrations in the CD-1 mouse. Toxicol Appl Pharmacol 1982;66:338-48. |
|16.||Warkany J, Kalter H. Maternal impressions and congenital malformations. Plast Reconstr Surg Transplant Bull 1962;30:628-37. |
|17.||Sanocka D, Kurpisz M. Reactive oxygen species and sperm cells. Reprod Biol Endocrinol 2004;2:12. |
|18.||Agarwal A, Nallella KP, Allamaneni SS, Said TM. Role of antioxidants in treatment of male infertility: An overview of the literature. Reprod Biomed Online 2004;8:616-27. |
|19.||Rajeswary S, Mathew N, Akbarsha MA, Kalyanasundram M, Kumaran B. Protective effect of vitamin E against carbendazim-induced testicular toxicity-histopathological evidences and reduced residue levels in testis and serum. Arch Toxicol 2007;81:813-21. |
|20.||Helmstetter FJ, Bellgowan PS. Hypoalgesia in response to sensitization during acute noise stress. Behav Neurosci 1994;108:177-85. |
|21.||Sarkaki A, Karami K. Impaired learning due to noise stress during pregnancy in rats offspring. J Res Med Sci2004;9:275-9. |
|22.||Sarkaki A, Heydari A, Shahraki M. Effects of noise stress during fetal life on pain threshold in rats. J Kerman Univ Med Sci 2000;7:53-9. |
|23.||Chandralekha G. The effect of noise induced stress on the male reproductive endocrine glands of Albino rats. Ph. D thesis. Chennai: Dr. M.G.R. Medical University and Research; 2002. |
|24.||Swami CG, Ramanathan J, Jeganath CC. Noise exposure effect on testicular histology, morphology and on male steroidogenic hormone. Malaysian J Med Sci 2007;14:28-35. |
|25.||Armario A, Castellanos JM. Effects of noise stress on testosterone secretion in mice. IRCS Med Sci 1984;12:208-10. |
|26.||Rivier C, Rivest S. Effect of stress on the activity of the hypothalamic-pituitary-gonadal axis: Peripheral and central mechanisms. Biol Reprod 1991;45:523-32. |
|27.||Almeida SA, Petenusci SO, Anselmo-Franci JA, Rosa-e-Silva AA, LamanoCarvalho TL. Chronic intermittent immobilization of male rats throughout sexual development: A stress protocol. Brazilian J Med Biol Res 1998;31:1443-8. |
|28.||López-Calderón A, Ariznavarreta C, González-Quijano MI, Tresguerres JA, Calderón MD. Stress induced changes in testis function. J Steroid Biochem Mol Biol 1991;40:473-9. |
|29.||Nawrot PS, Cook RO, Hamm CW. Embryotoxicity of broadband high-frequency noise in the CD-1 mouse. J Toxicol Environ Health 1981;8:151-7. |
|30.||Sies H, Stahl W, Sundquist AR. Antioxidant functions of Vitamins. Vitamins E and C, beta-carotene, and other carotenoids. Ann N Y Acad Sci 1992;669:7-20. |
|31.||Lewis SE, Sterling ES, Young IS, Thompson W. Comparison of individual antioxidants of sperm and seminal plasma in fertile and infertile men. Fertil Steril 1997;67:142-7. |
|32.||Akmal M, Qadri JQ, Al-Waili NS, Thangal S, Haq A, Saloom KY. Improvement in human semen quality after oral supplementation of vitamin C. J Med Food 2006;9:440-2. |
|33.||Kessopoulou E, Powers HJ, Sharma KK, Pearson MJ, Russell JM, Cooke ID, et al. A double-blind randomized placebo cross-over controlled trial using the antioxidant vitamin E to treat reactive oxygen species associated male infertility. Fertil Steril 1995;64:825-31. |
|34.||Günther E. Animal experimental studies on the effects of noise stress on male fertility. Andrologia 1976;8:95-100. |
Physiology Research center, Ahvaz Joundishapour University, Ahvaz
Source of Support: Ahvaz Jundishapur University of Medical Sciences, Conflict of Interest: None
[Table 1], [Table 2]
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