Home Email this page Print this page Bookmark this page Decrease font size Default font size Increase font size
Noise & Health  
 CURRENT ISSUE    PAST ISSUES    AHEAD OF PRINT    SEARCH   GET E-ALERTS    
 
 Next article
 Previous article
Table of Contents

Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
Citation Manager
Access Statistics
Reader Comments
Email Alert *
Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed6008    
    Printed193    
    Emailed2    
    PDF Downloaded114    
    Comments [Add]    

Recommend this journal

 

 ARTICLES
Year : 2001  |  Volume : 3  |  Issue : 11  |  Page : 1--18

Gene expression changes in chinchilla cochlea from noise-induced temporary threshold shift


Center for Hearing and Deafness, SUNY University of Buffalo, Buffalo, NY, USA

Correspondence Address:
Richard Salvi
Hearing Research Lab, 215 Parker Hall, SUNY University of Buffalo, Buffalo, NY 14214
USA
Login to access the Email id

Source of Support: None, Conflict of Interest: None


PMID: 12689445

Rights and PermissionsRights and Permissions

Acoustic overstimulation produces many anatomical, biochemical and physiological changes in the inner ear. However, the changes in gene expression that underlie these biological changes are poorly understood. Our approach to investigating this problem is to use gene microarrays to measure the changes in gene expression in the chinchilla inner ear following a 3 h or 6 h noise exposure (95 dB SPL, 707-1414 Hz). This noise exposure causes a temporary threshold shift (~40 dB) and a temporary reduction in distortion product otoacoustic emissions (DPOAE), but no permanent hearing loss or hair cell loss. Here, we present data showing (1) the suitability of mouse and human complementary DNA (cDNA) clones for detecting chinchilla cochlear gene transcripts, and (2) the change in cochlear gene transcripts in noise exposed chinchillas. Chinchilla cochlear transcript probes exhibited strong and discrete signals on both mouse and human cDNA filter arrays. Since the strongest hybridization occurred with mouse clones, mouse cDNA microarrays were used to study noise-induced changes in gene expression. Chinchilla cDNA probes were differentially labelled with Cy3 (control) or Cy5 (noise exposed) by random primed synthesis, hybridized to 8750 mouse cDNAs arrayed on microscope slides and analysed by laser fluorescent microscopy. Several classes of genes exhibited time-dependent up regulation of transcription, including those involved in protein synthesis, metabolism, cytoskeletal proteins, and calcium binding proteins. The results are discussed in relationship to previous studies showing noise-induced changes in structural proteins, calcium binding proteins, metabolic enzymes and membrane bound vesicles.






[FULL TEXT] [PDF]*


        
Print this article     Email this article