| ORIGINAL ARTICLE |
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| Year : 2017 | Volume
: 19
| Issue : 87 | Page : 79--83 |
The Effects of Low-Frequency Noise on Rats: Evidence of Chromosomal Aberrations in the Bone Marrow Cells and the Release of Low-Molecular-Weight DNA in the Blood Plasma
Irina N Vasilyeva1, Vladimir G Bespalov1, Alexander L Semenov1, Denis A Baranenko2, Valery N Zinkin3
1 Scientific Laboratory for Cancer Chemoprevention and Oncopharmacology at N.N. Petrov Research Institute of Oncology under the Ministry of Health of the Russian Federation, Moscow; International Research Centre “Biotechnologies of the Third Millennium”, ITMO University, St. Petersburg, Russian Federation
2 International Research Centre “Biotechnologies of the Third Millennium”, ITMO University, St. Petersburg, Russian Federation
3 Research and Testing Center of Aerospace Medicine and Military Ergonomics at 4th Central Research Institute under the Ministry of Defence of the Russian Federation, Moscow, Russian Federation
Correspondence Address:
Irina N Vasilyeva
Scientific Laboratory for Cancer Chemoprevention and Oncopharmacology at N.N. Petrov Research Institute of Oncology under the Ministry of Health of the Russian Federation, Leningradskaya str. 68, Pesochny, 197758 St. Petersburg
Russian Federation
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/nah.NAH_39_16
Objectives: Evaluation of the effect of low-frequency noise (LFN) on the frequency of chromosomal aberrations in the bone marrow cells and on the content of low-molecular-weight DNA (lmwDNA) in the blood plasma of rats. Materials and Methods: A total of 96 male Wistar rats were exposed to either single (17 min session) or multiple (17 min session repeated five times a week for 13 weeks) LFN, with the maximum range below 250 Hz and the sound pressure levels (SPLs) at 120 and 150 dB, respectively. The rats in the control groups were not subjected to any impact. The frequency of chromosomal aberrations in the bone marrow cells and the levels of lmwDNA in the blood plasma were measured afterwards. Results: It has been detected that a single LFN exposure with either corresponding SPLs had a significant increase in the frequency of chromosomal aberrations (more than 10-fold) compared to the controls (0.9 ± 0.3%) and resulted in the appearance of dicentric chromosomes in the aberration spectrum, both of which are evident for the occurrence of deoxyribonucleic acid double strand breaks triggered by the exposure. Furthermore, the lmwDNA levels in the blood plasma measured the following day after a single LFN exposure were significantly higher (7.7- and 7.6-fold, respectively) than that in the control group (11.0 ± 5.4 ng/ml), and such levels were maintained higher (4.8- and 2.1-fold, respectively) in the week after a single LFN exposure for the SPL of 120 and 150 dB, respectively, compared to the control group (18.8 ± 1.6 ng/ml). Similar results were obtained from the group with multiple LFN exposures (36.4- and 22.4-fold, respectively) compared to the control (17.7 ± 1.7 ng/ml) and suggest the enhancement of cellular apoptosis as a result of the LFN impact. Conclusion: Presumably, the LFN may have possible mutagenic effects and cause massive cell death.
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