Figure 4: Difference in binding of Oua-Bodipy (OUABDP) to the Na-K-ATPase of MCF7 cells after exposure to the speakers alone or to the three compositions. The cells were incubated for 30 min with OUABDP in complete silence (computed tomography [CT] OUABDP) or in the presence of speaker alone or music, as described in material and methods. After this time, the cells were harvested by trypsin and the fluorescence of the cells was measured. (a) Top panel-auto fluorescence and bottom panel - CT OUABDP. The region R1 was the gate used to compare the control (cells remained in silence) with the cells exposed to the speakers or music; (b) Graphs representing the percentage of cells gated in Region R1. ^aStatistically different in relation to the control cells remained in silence (CT OUABDP), as measured by the Student's t test (P < 0.01); (c) Comparison of cells treated with music with those exposed to the speaker alone. ^aStatistically different in relation to the speaker, as measured by ANOVA followed by Dunnett's multiple comparison test (P < 0.05)