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Figure 3: Microscopic analysis of the distribution of BiP/GRP78 and CHOP/Gadd153 in immunohistochemically stained cochleae (SP, ×400). (a-1–a-3) Cells that were positively stained for BiP/GRP were located in the organ of Corti, the lateral wall, and the spiral ganglion, respectively, in the 1d group (positive expression: immuoreactivity is brown versus the blue hematoxylin stain, indicated by ↑). (b-1–b-3) Cells that were positively stained for CHOP/Gadd153 were located in the same regions as those shown in (a), in the 1d group (positive expression: immunoreactivity is tan or medium-brown particles versus hematoxylin-stain, indicated by ↑). (c-1–c-3) Negative control incubated with PBS instead of the primary antibodies (organ of Corti, the lateral wall, and the spiral ganglion, respectively)

Figure 3: Microscopic analysis of the distribution of BiP/GRP78 and CHOP/Gadd153 in immunohistochemically stained cochleae (SP, ×400). (a-1–a-3) Cells that were positively stained for BiP/GRP were located in the organ of Corti, the lateral wall, and the spiral ganglion, respectively, in the 1d group (positive expression: immuoreactivity is brown versus the blue hematoxylin stain, indicated by ↑). (b-1–b-3) Cells that were positively stained for CHOP/Gadd153 were located in the same regions as those shown in (a), in the 1d group (positive expression: immunoreactivity is tan or medium-brown particles versus hematoxylin-stain, indicated by ↑). (c-1–c-3) Negative control incubated with PBS instead of the primary antibodies (organ of Corti, the lateral wall, and the spiral ganglion, respectively)