Figure 2: Effects of infrasound on the levels of miR-29a, phosphorylated Smad3, and TGF-β in the cultured cardiac fibroblasts stimulated by angiotensin II. (a) Levels of miR-29a detected by real-time PCR. (b) Phosphorylation status of Smad3 was determined by western blotting with total Smad3 as internal control. (c) TGF-β in the supernatant was detected by ELISA. The results were presented as times of control, and the data were from three independent experiments. ^*P < 0.05, ^**P < 0.01 versus control group; ^#P < 0.05, ^##P < 0.01 versus Ang II group