Figure 4: Levels of phosphorylated Smad3, and TGF-β in the siRNA or control cells treated with Ang II with or without exposure to infrasound. (a) Phosphorylation status of Smad3 was determined by western blotting with total Smad3 as internal control. (b) TGF-β in the supernatant was detected by ELISA. The results were presented as times of control, and the data were from three independent experiments. ^*P < 0.05, ^**P < 0.01 versus control group; ^#P < 0.05 versus Ang II group